The Whole Number Rule states that the masses of the elements are whole number multiples of the mass of the hydrogen atom.1 The rule can be formulated from Prout's hypothesis put forth in 1815.2 In 1920, Francis W. Aston demonstrated through the use of a mass spectrometer that apparent deviations from the rule were due to the existence of isotopes.3
^ Budzikiewicz H, Grigsby RD (2006). "Mass spectrometry and isotopes: a century of research and discussion". Mass spectrometry reviews25 (1): 146–57. doi:10.1002/mas.20061. PMID 16134128.
^ Aston, Francis W. (1920). "The constitution of atmospheric neon". Philosophical Magazine39 (6): 449–455.
Harkins WD (1925). "The Separation of Chlorine into Isotopes (Isotopic Elements) and the Whole Number Rule for Atomic Weights". Proc. Natl. Acad. Sci. U.S.A.11 (10): 624–8. PMID 16587053.