Selectins
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P selectin lectin bound to sugar, shown in sticks, PDB 1G1R

Selectins are a family of cell adhesion molecules (or CAMs). All selectins are single-chain transmembrane glycoproteins which share similar properties to C-type lectins due to a related amino terminus and calcium-dependent binding^[1]. Selectins bind to sugar moieties and so are considered to be a type of lectin, cell adhesion proteins that bind sugar polymers.^[2]

Types

There are three subsets of selectins:

• E-selectin (in endothelial cells)
• L-selectin (in leukocytes)
• P-selectin (in platelets and endothelial cells)

Etymology

The name selectin comes from the words "selected" and "lectins" which are a type of carbohydrate recognizing proteins.

Function

During an inflammatory response stimuli such as histamine and thrombin cause endothelial cells to mobilize P-selectin from stores inside the cell to the cell surface. In addition, cytokines such as TNF-alpha stimulate the expression of E-selectin and additional P-selectin a few hours later.

As the leukocyte rolls along the blood vessel wall, the distal lectin-like domain of the selectin binds to certain carbohydrate groups presented on proteins (such as PSGL-1) on the leukocyte, which slows the cell and allows it to leave the blood vessel and enter the site of infection. The low-affinity nature of selectins is what allows the characteristic "rolling" action attributed to leukocytes during the leukocyte adhesion cascade^[1].

The best-characterized ligand for the three selectins is P-selectin glycoprotein ligand-1 (PSGL-1), which is a mucin-type glycoprotein expressed on all white blood cells.

Neutrophils and eosinophils bind to E-selectin. One of the reported ligands for E-selectin is the sialylated Lewis X Ag (sLe(x)). Eosinophils, like neutrophils, use sialylated, protease-resistant structures to bind to E-selectin, although the eosinophil expresses much lower levels of these structures on its surface. ^[3] Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase-resistant structures, clearly different than those reported for E-selectin, and suggest disparate roles for P-selectin and E-selectin during recruitment during inflammatory responses. ^[4]

Research

Selectins are involved in significant biomedical research. One project involves using selectins in nanodevices to treat cancer. Researchers are trying to create a device capable of killing cancer cells circulating in the blood^[5][6]. The scientists have covalently attached selectins to an epoxy surface in order to encourage tumor cells and other cells to roll. Also attached to the surface is a ligand that selectively signals cancer cells to undergo apoptosis, or cell death. Without selectins, the device would be unable to slow cancer cells down and would thus be unable to kill them.

Selectins are also involved in projects to treat osteoporosis, a disease that occurs when bone-creating cells called osteoblasts become too scarce. Osteoblasts develop from stem cells, and scientists hope to eventually be able to treat osteoporosis by adding stem cells to a patient’s bone marrow. Researchers have developed a way to use selectins to direct stem cells introduced into the vascular system to the bone marrow^[7]. E-selectins are constitutively expressed in the bone marrow, and researchers have shown that tagging stem cells with a certain glycoprotein causes these cells to migrate to the bone marrow. Thus, selectins may someday be essential to a regenerative therapy for osteoporosis^[8].

External links

• CIMIT Center for Integration of Medicine and Innovative Technology
• Karp Lab on Research
• Sackstein Lab of Research
• Computer-generated movie of the mobilization of P-selectin inside a leukocyte at mcb.harvard.edu

References

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