Oil Red O
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Oil Red O
Identifiers
CAS number	[1320-06-5]
PubChem	5841742
MeSH	oil+red+O
SMILES	CC1=C(C(=CC=C1)N=NC2=C(C(=C(C=C2)NN=C3C(=O)C=CC4=CC=CC=C43)C)C)C
Properties
Molecular formula	C26H24N4O
Molar mass	408.49496
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox references

Oil Red O (Solvent Red 27, Sudan Red 5B, C.I. 26125, C₂₆H₂₄N₄O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a red powder with maximum absorption at 518(359) nm.

Its CAS number is 1320-06-5 and its SMILES structure is OC1=C(N=NC3=CC(C) =C(N=NC4=CC(C) =CC=C4C)C=C3C) C2=C(C=CC=C2)C=C1.

Uses

Oil Red O is one of the dyes used for Sudan staining. Similar dyes include Sudan III, Sudan IV, and Sudan Black B. The staining has to be performed on fresh samples, as alcohol fixation removes the lipids.

Oil Red O largely replaced Sudan III and Sudan IV, as it provides much deeper red color and the stains are therefore much easier to see.

When staining, it can make fat more visible.^[1]

In pyrotechnics, Oil Red O is used in some compositions of red colored smokes. It is also used to dye some plastics, eg. polystyrene resins.

References

External links

• Stains File entry

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