Occludin
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Occludin".

content
Occludin
PDB, Structure of the C-Terminus of Occludin
Available structures: 1wpa, 1xaw
Identifiers
Symbols OCLN;
External IDs OMIM: 602876 MGI: 106183 HomoloGene: 1905
Orthologs
Human Mouse
Entrez 4950 18260
Ensembl n/a ENSMUSG00000021638
Uniprot n/a Q3UKZ5
Refseq XM_001128133 (mRNA)
XP_001128133 (protein)		 NM_008756 (mRNA)
NP_032782 (protein)
Location n/a Chr 13: 101.6 - 101.65 Mb
Pubmed search [1] [2]

Occludin, also known as OCLN, is a human gene.1

Occludin is a 65-kDa (504-amino acid polypeptide) integral plasma-membrane protein located specifically at tight junctions described for the first time in 1993 by S Tsukita.2

Contents

Structure

According to its overall hydrophilicity, occludin appears to span the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol. Interaction of occludin with several cytoplasmic proteins of the junctional plaque has been found to occur via its COOH terminus, while the extracellular loops are thought to be involved in the regulation of paracellular permeability and cell adhesion. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions.

Disease linkage

Disruption of occludin regulation is an important aspect of a number of diseases. Strategies to prevent and/or reverse occludin downregulation may be an important therapeutic target.

Diagram of Tight junction.

See also

References

  1. ^ "Entrez Gene: OCLN occludin".
  2. ^ Furuse M, Hirase T, Itoh M, Nagafuchi A, Yonemura S, Tsukita S, Tsukita S (1993). "Occludin: a novel integral membrane protein localizing at tight junctions". J. Cell Biol. 123 (6 Pt 2): 1777–88. doi:10.1083/jcb.123.6.1777. PMID 8276896. 

Further reading

  • Furuse M, Itoh M, Hirase T, et al. (1994). "Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions.". J. Cell Biol. 127 (6 Pt 1): 1617–26. PMID 7798316. 
  • Ando-Akatsuka Y, Saitou M, Hirase T, et al. (1996). "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues.". J. Cell Biol. 133 (1): 43–7. PMID 8601611. 
  • Van Itallie CM, Anderson JM (1997). "Occludin confers adhesiveness when expressed in fibroblasts.". J. Cell. Sci. 110 ( Pt 9): 1113–21. PMID 9175707. 
  • Kimura Y, Shiozaki H, Hirao M, et al. (1997). "Expression of occludin, tight-junction-associated protein, in human digestive tract.". Am. J. Pathol. 151 (1): 45–54. PMID 9212730. 
  • Saitou M, Ando-Akatsuka Y, Itoh M, et al. (1997). "Mammalian occludin in epithelial cells: its expression and subcellular distribution.". Eur. J. Cell Biol. 73 (3): 222–31. PMID 9243183. 
  • Haskins J, Gu L, Wittchen ES, et al. (1998). "ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin.". J. Cell Biol. 141 (1): 199–208. PMID 9531559. 
  • Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM (1998). "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton.". J. Biol. Chem. 273 (45): 29745–53. PMID 9792688. 
  • Itoh M, Morita K, Tsukita S (1999). "Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin.". J. Biol. Chem. 274 (9): 5981–6. PMID 10026224. 
  • Jiang WG, Martin TA, Matsumoto K, et al. (1999). "Hepatocyte growth factor/scatter factor decreases the expression of occludin and transendothelial resistance (TER) and increases paracellular permeability in human vascular endothelial cells.". J. Cell. Physiol. 181 (2): 319–29. doi:10.1002/(SICI)1097-4652(199911)181:2<319::AID-JCP14>3.0.CO;2-S. PMID 10497311. 
  • Wittchen ES, Haskins J, Stevenson BR (2000). "Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3.". J. Biol. Chem. 274 (49): 35179–85. PMID 10575001. 
  • Kojima T, Sawada N, Chiba H, et al. (2000). "Induction of tight junctions in human connexin 32 (hCx32)-transfected mouse hepatocytes: connexin 32 interacts with occludin.". Biochem. Biophys. Res. Commun. 266 (1): 222–9. doi:10.1006/bbrc.1999.1778. PMID 10581193. 
  • Burns AR, Bowden RA, MacDonell SD, et al. (2000). "Analysis of tight junctions during neutrophil transendothelial migration.". J. Cell. Sci. 113 ( Pt 1): 45–57. PMID 10591624. 
  • Itoh M, Furuse M, Morita K, et al. (2000). "Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins.". J. Cell Biol. 147 (6): 1351–63. PMID 10601346. 
  • Singh U, Van Itallie CM, Mitic LL, et al. (2000). "CaCo-2 cells treated with Clostridium perfringens enterotoxin form multiple large complex species, one of which contains the tight junction protein occludin.". J. Biol. Chem. 275 (24): 18407–17. doi:10.1074/jbc.M001530200. PMID 10749869. 
  • Marzioni D, Banita M, Felici A, et al. (2001). "Expression of ZO-1 and occludin in normal human placenta and in hydatidiform moles.". Mol. Hum. Reprod. 7 (3): 279–85. PMID 11228248. 
  • Andreeva AY, Krause E, Müller EC, et al. (2001). "Protein kinase C regulates the phosphorylation and cellular localization of occludin.". J. Biol. Chem. 276 (42): 38480–6. doi:10.1074/jbc.M104923200. PMID 11502742. 
  • Papadopoulos MC, Saadoun S, Woodrow CJ, et al. (2001). "Occludin expression in microvessels of neoplastic and non-neoplastic human brain.". Neuropathol. Appl. Neurobiol. 27 (5): 384–95. PMID 11679090. 
  • Schmidt A, Utepbergenov DI, Krause G, Blasig IE (2001). "Use of surface plasmon resonance for real-time analysis of the interaction of ZO-1 and occludin.". Biochem. Biophys. Res. Commun. 288 (5): 1194–9. doi:10.1006/bbrc.2001.5914. PMID 11700038. 
  • Pummi K, Malminen M, Aho H, et al. (2001). "Epidermal tight junctions: ZO-1 and occludin are expressed in mature, developing, and affected skin and in vitro differentiating keratinocytes.". J. Invest. Dermatol. 117 (5): 1050–8. doi:10.1046/j.0022-202x.2001.01493.x. PMID 11710912. 
  • Traweger A, Fang D, Liu YC, et al. (2002). "The tight junction-specific protein occludin is a functional target of the E3 ubiquitin-protein ligase itch.". J. Biol. Chem. 277 (12): 10201–8. doi:10.1074/jbc.M111384200. PMID 11782481. 

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