Nucleic acid methods
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Nucleic_acid_methods".

Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).

Purification

• Phenol-chloroform extraction
• minicolumn purification

Quantification

• Abundance in weight: spectroscopic quantification
• Absolute abundance in number: RTPCR
• high-throughput relative abundance DNA microarray
• high-throughput absolute abundance SAGE
• Size: Gel electrophoresis

Synthesis

• De novo: Oligonucleotide synthesis
• Amplification: PCR

Other

• DNA sequencing
• Bisulfite sequencing
• Expression cloning
• Southern blot
• northern blot
• Sucrose gradient centrifugation
• Radioactivity in biological research
• Lab-on-a-chip
• Nuclear run-on assay
• Fluorescent in situ hybridization
• several Bioinformatics methods, such as RNA structure prediction
  
  

Protocols and other How-to information are not present in Wikipedia (see WP:NOT#HOWTO), for these one could consult Open wetware website