Unpolarized light enters one end of the crystal and is split into two polarized rays by birefringence. One of these rays (the ordinary or o-ray) experiences a refractive index of no = 1.658 and at the balsam layer (refractive index n = 1.55) undergoes total internal reflection at the interface, and is reflected to the side of the prism. The other ray (the extraordinary or e-ray) experiences a lower refractive index (ne = 1.486), is not reflected at the interface, and leaves through the second half of the prism as plane polarized light.
Nicol prisms were once widely used in microscopy and polarimetry, and the term "crossed Nicols" (abbreviated as XN) is still used to refer to observation of a sample between orthogonally orientated polarizers. In most instruments, however, Nicol prisms have been supplanted by other types of polarizers such as Polaroid sheets and Glan-Thompson prisms.
