The duet "Sul suo capo aggravi un Dio" between Anna (soprano) and Jane Seymour (mezzo soprano) is considered one of the finest in the entire operatic repertoire.
Anna Bolena was performed infrequently during the latter half of 19th century and early 20th century, but it was revived with more frequency in the post-war years. On December 30, 1947, the opera was performed at Gran Teatre del Liceu in Barcelona, celebrating the centennial of this theatre, which opened in 1847 with Anna Bolena. The cast was Sara Scuderi as Anna Bolena, Giulietta Simionato as Jane Seymour and Cesare Siepi as Henry VIII.