Amidase
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Amidase".

In enzymology, an amidase (EC 3.5.1.4) is an enzyme that catalyzes the chemical reaction

a monocarboxylic acid amide + H₂O a monocarboxylate + NH₃

Thus, the two substrates of this enzyme are monocarboxylic acid amide and H₂O, whereas its two products are monocarboxylate and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is acylamide amidohydrolase. Other names in common use include acylamidase, acylase, amidohydrolase, deaminase, fatty acylamidase, and N-acetylaminohydrolase. This enzyme participates in 6 metabolic pathways: urea cycle and metabolism of amino groups, phenylalanine metabolism, tryptophan metabolism, cyanoamino acid metabolism, benzoate degradation via coa ligation, and styrene degradation.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2PLQ and 2UXY.

References

• IUBMB entry for 3.5.1.4
• BRENDA references for 3.5.1.4 (Recommended.)
• PubMed references for 3.5.1.4
• PubMed Central references for 3.5.1.4
• Google Scholar references for 3.5.1.4
• Bray HG, James SP, Raffan IM, Ryman BE and Thorpe WV (1949). "The fate of certain organic acids and amides in the rabbit. 7. An amidase of rabbit liver". Biochem. J. 44: 618–625. 
• Bray HG, James SP, Thorpe WV and Wasdell MR (1950). "The fate of certain organic acids and amides in the rabbit. 11 Further observations on the hydrolysis of amides by tissue extracts". Biochem. J. 47: 294–299. 

External links

The CAS registry number for this enzyme class is 9012-56-0.

• IUBMB entry for 3.5.1.4

• KEGG entry for 3.5.1.4

• BRENDA entry for 3.5.1.4

• NiceZyme view of 3.5.1.4

• EC2PDB: PDB structures for 3.5.1.4

• PRIAM entry for 3.5.1.4

• PUMA2 entry for 3.5.1.4

• IntEnz: Integrated Enzyme entry for 3.5.1.4

• MetaCyc entry for 3.5.1.4

• Atomic-resolution structures of enzymes belonging to this class

Gene Ontology (GO) codes

• EGO entry for GO code 0004040: amidase

• AMIGO entry for GO code 0004040: amidase

This hydrolase article is a stub. You can help Wikipedia by expanding it.