Allan Maxam
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Allan Maxam

Doctoral advisor Walter Gilbert
Known for DNA sequencing

Allan Maxam is one of the pioneers of molecular genetics. He was one of the contributors to develop a DNA sequencing method at Harvard University, while working as a student in the laboratory of Walter Gilbert [1][2].

Walter Gilbert and Allan Maxam developed a DNA sequencing method which combined chemicals that cut DNA only at specific bases with radioactive labeling and Southern blotting to determine the sequence of long DNA segments[3].

References

  1. ^ Maxam, A M & Gilbert, W (1977), “A new method for sequencing DNA.”, Proc. Natl. Acad. Sci. U.S.A. 74 (2): 560-4, 1977 Feb, PMID:265521, <http://www.ncbi.nlm.nih.gov/pubmed/265521> 
  2. ^ DNA SEQUENCING AND GENE STRUCTURE, Walter Gilbert Nobel lecture, 8 December, 1980
  3. ^ Maxam AM, Gilbert W, Sequencing end-labeled DNA with base-specific chemical cleavages, Methods Enzymol. 1980;65(1):499-560
  • Gilbert, Walter and Maxam. Allan, The Nuclcotide Sequence of the Lac Operator, Proc. Natl. Acad. Sci. USA 70, 3581-3584 (1973).
  • Maxam AM, Tizard R, Skryabin KG, Gilbert W, Promotor region for yeast 5S ribosomal RNA, Nature. 1977 Jun 16;267(5612):643-5

See also

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