2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase".

In enzymology, a 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12) is an enzyme that catalyzes the chemical reaction

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP

Hence, this enzyme has one substrate, 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol, and two products, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate and CMP.

This enzyme belongs to the family of lyases, specifically phosphorus-oxygen lyases. The systematic name of this enzyme class is 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol CMP-lyase (cyclizing; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate-forming). Other names in common use include MECDP-synthase, 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol, and CMP-lyase (cyclizing). This enzyme participates in biosynthesis of steroids.

Structural studies

As of late 2007, 20 structures have been solved for this class of enzymes, with PDB accession codes 1GX1, 1H47, 1H48, 1IV1, 1IV2, 1IV3, 1IV4, 1T0A, 1U3L, 1U3P, 1U40, 1U43, 1VH8, 1VHA, 1W55, 1W57, 1YQN, 2AMT, 2GZL, and 2PMP.

References

• IUBMB entry for 4.6.1.12
• BRENDA references for 4.6.1.12 (Recommended.)
• PubMed references for 4.6.1.12
• PubMed Central references for 4.6.1.12
• Google Scholar references for 4.6.1.12
• Fellermeier M, Eisenreich W, Zenk MH, Bacher A, Rohdich F (2000). "Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate". Proc. Natl. Acad. Sci. U. S. A. 97: 2486–90. doi:10.1073/pnas.040554697. PMID 10694574. 
• Seto H (2000). "Studies on the nonmevalonate pathway: Formation of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate from 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol". Tetrahedron Lett. 41: 3395–3398. doi:10.1016/S0040-4039(00)00375-0. 

External links

• IUBMB entry for 4.6.1.12

• KEGG entry for 4.6.1.12

• BRENDA entry for 4.6.1.12

• NiceZyme view of 4.6.1.12

• EC2PDB: PDB structures for 4.6.1.12

• PRIAM entry for 4.6.1.12

• PUMA2 entry for 4.6.1.12

• IntEnz: Integrated Enzyme entry for 4.6.1.12

• MetaCyc entry for 4.6.1.12

• Atomic-resolution structures of enzymes belonging to this class

Gene Ontology (GO) codes

• EGO entry for GO code 0008685: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

• AMIGO entry for GO code 0008685: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

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